Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2013-11-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 61 |
| Unit cell lengths | 78.560, 78.560, 224.801 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.329 - 3.101 |
| R-factor | 0.2288 |
| Rwork | 0.225 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.914 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1448)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 13924 | |
| Completeness [%] | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.1 M HEPES, pH 7.4, 5% PEG6000, 5% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
