4OI4
Protein complex of Clp1 bound to ATP and Mg2+ with Pcf11deltaN454deltaC563 of S. cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9789 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.640, 95.770, 181.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 2.400 |
R-factor | 0.18873 |
Rwork | 0.187 |
R-free | 0.22133 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2npi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.244 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 61162 | |
Completeness [%] | 97.9 | 97.9 |
Redundancy | 5.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 16% w/v PEG6000, 100 mM Tris-HCl, pH 8.5, 18% v/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |