4OHT

Crystal structure of succinic semialdehyde dehydrogenase from Streptococcus pyogenes in complex with NADP+ as the cofactor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97951
Spacegroup name	P 43 21 2
Unit cell lengths	134.759, 134.759, 173.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.890 - 2.100
R-factor	0.201
Rwork	0.201
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ogd
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.126	0.441
Number of reflections	93776
<I/σ(I)>	21.5	3.7
Completeness [%]	99.4	98.7
Redundancy	8.9	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	0.1 M sodium acetate trihydrate and 2.0 M ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K