4OHD
LEOPARD Syndrome-Associated SHP2/A461T mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-17 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 54.310, 206.650, 41.881 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.814 - 2.700 |
R-factor | 0.2059 |
Rwork | 0.202 |
R-free | 0.27080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dgp |
RMSD bond length | 0.009 |
RMSD bond angle | 1.186 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.122 | 0.309 |
Number of reflections | 68055 | |
<I/σ(I)> | 18.738 | 4.167 |
Completeness [%] | 94.9 | 74.1 |
Redundancy | 5.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 18% PEG3350, 300 mM KF, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |