4OH8
Crystal Structure of the human MST1-RASSF5 SARAH heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 27.940, 85.840, 92.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.093 - 2.281 |
R-factor | 0.2195 |
Rwork | 0.217 |
R-free | 0.27780 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.122 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.079 | 0.206 |
Number of reflections | 22807 | |
<I/σ(I)> | 31.733 | 6.031 |
Completeness [%] | 96.9 | 85.8 |
Redundancy | 4 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 35% (v/v) 2-methyl-2,4-pentanediol (MPD) acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |