Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

4OGY

Crystal structure of Fab DX-2930 in complex with human plasma kallikrein at 2.1 Angstrom resolution

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU FR-E+ SUPERBRIGHT
Temperature [K]100
Detector technologyCCD
Collection date2013-02-22
DetectorRIGAKU SATURN 944+
Wavelength(s)1.5418
Spacegroup nameP 1 21 1
Unit cell lengths82.120, 113.760, 89.050
Unit cell angles90.00, 94.70, 90.00
Refinement procedure
Resolution40.590 - 2.100
R-factor0.185
Rwork0.183
R-free0.22100
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2ANY AND 3IDX
RMSD bond length0.011
RMSD bond angle1.361
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwareREFMAC (5.7.0032)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.140
High resolution limit [Å]2.1002.100
Rmerge0.0760.346
Number of reflections948971098
<I/σ(I)>22.65.8
Completeness [%]99.898.8
Redundancy7.36.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP6.5289FAB DX-2930 AND HUMAN PLASMA KALLIKREIN VCID 7481 AT 150 UM OR 11.7 MG/ML AGAINST INDEX screen condition D10, 20% PEG 5000 MME, 0.1 M BisTris pH 6.5 supplemented with 20% ethylene glycol as cryo-protectant, unique puck ID qjo1-15, crystal tracking ID 242121d10, VAPOR DIFFUSION, SITTING DROP, temperature 289K

226707

PDB entries from 2024-10-30

PDB statisticsPDBj update infoContact PDBjnumon