4OGC

Crystal structure of the Type II-C Cas9 enzyme from Actinomyces naeslundii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.116
Spacegroup name	P 1 21 1
Unit cell lengths	74.610, 132.560, 80.040
Unit cell angles	90.00, 95.38, 90.00

Refinement procedure

Resolution	68.297 - 2.800
R-factor	0.1962
Rwork	0.194
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.808
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.690	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	38240
Completeness [%]	100.0	100
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	10% (w/v) PEG 8000, 0.25 M calcium acetate, 50 mM magnesium acetate and 5 mM spermidine., pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	10% (w/v) PEG 8000, 0.25 M calcium acetate, 50 mM magnesium acetate and 5 mM spermidine., pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K