4OF5
Refinement of RAGE-DNA complex in 3S59 without DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0332 |
Spacegroup name | P 61 |
Unit cell lengths | 79.121, 79.121, 224.045 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.560 - 2.803 |
R-factor | 0.2208 |
Rwork | 0.220 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3S59 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.627 |
Refinement software | PHENIX (1.8.3_1479) |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 10% PEG6000, 0.1 M Tris_HCl pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |