4ODV
Fab Structure of lipid A-specific antibody A6 in complex with lipid A carbohydrate backbone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.563, 64.285, 154.157 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.830 - 2.150 |
R-factor | 0.2067 |
Rwork | 0.204 |
R-free | 0.24880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4odt |
RMSD bond length | 0.011 |
RMSD bond angle | 1.564 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 2.230 |
High resolution limit [Å] | 2.150 | 4.620 | 2.150 |
Rmerge | 0.085 | 0.061 | 0.404 |
Number of reflections | 21071 | ||
<I/σ(I)> | 12.8 | ||
Completeness [%] | 99.7 | 99.5 | 99.1 |
Redundancy | 4.9 | 4.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M HEPES pH 7.5, 25% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |