4OBV
Ruminococcus gnavus tryptophan decarboxylase RUMGNA_01526 (alpha-FMT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1159 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 135.030, 135.030, 249.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.442 - 2.840 |
R-factor | 0.211 |
Rwork | 0.210 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.543 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Refinement software | PHENIX ((phenix.refine: dev_1260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.442 | 2.991 |
High resolution limit [Å] | 2.840 | 2.840 |
Number of reflections | 55229 | |
Completeness [%] | 99.9 | 99.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 298 | 30% ethoxyethanol, 0.1M citrate pH 5.25, 4% polypropylene P400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |