4O9L
crystal structure of horse MAVS card domain mutant E26R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2012-06-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 50.614, 51.942, 35.754 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.203 - 1.944 |
R-factor | 0.1646 |
Rwork | 0.163 |
R-free | 0.20920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vgq |
RMSD bond length | 0.007 |
RMSD bond angle | 0.943 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | CCP4 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 8395 | |
Completeness [%] | 91.5 | 55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% PEGMME 3350, 0.2M ammonium sulfate, 0.1M Mes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |