4O9H
Structure of Interleukin-6 in complex with a Camelid Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-10 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.210, 47.470, 148.320 |
| Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
| Resolution | 38.650 - 2.420 |
| R-factor | 0.217 |
| Rwork | 0.216 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.480 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.060 | 0.500 |
| Number of reflections | 28984 | |
| <I/σ(I)> | 14.2 | 2.2 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 277 | 2M Ammonium sulfate, 0.15M Na citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
