4O9F

crystal structure of horse MAVS card domain mutant R64C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2011-08-13
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5481
Spacegroup name	P 21 21 2
Unit cell lengths	50.816, 52.029, 35.579
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.354 - 2.348
R-factor	0.1666
Rwork	0.163
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vgq
RMSD bond length	0.007
RMSD bond angle	0.960
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	CCP4
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.348	2.350
Number of reflections	4212
Completeness [%]	98.8	84.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M Bis-Tris, 25% PEG 3350, 0.20 M ammonium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K