4O8Y

Zinc-free Rpn11 in complex with Rpn8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750
Spacegroup name	P 43 21 2
Unit cell lengths	70.405, 70.405, 198.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.615 - 1.950
R-factor	0.1652
Rwork	0.163
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4F70 2o95
RMSD bond length	0.013
RMSD bond angle	1.385
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.950	5.290	1.950
Rmerge	0.075	0.030
Number of reflections	37562
<I/σ(I)>	9.3
Completeness [%]	100.0	99.8	100
Redundancy	11.9	10.8	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	15% PEG8000, 30% ethylene glycol, and 100 mM HEPES (pH7.5), VAPOR DIFFUSION, HANGING DROP, temperature 291K