4O82
SAICAR synthetase (Type-1) in complex with ADP AND AMP in both chains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-11-05 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 |
Unit cell lengths | 94.980, 94.980, 147.701 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.530 - 2.160 |
R-factor | 0.235 |
Rwork | 0.233 |
R-free | 0.27340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u54 CHAIN A |
RMSD bond length | 0.009 |
RMSD bond angle | 1.418 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.230 | 2.280 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.052 | 0.276 |
Number of reflections | 26649 | |
<I/σ(I)> | 14.1 | 4.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch Underoil | 7.5 | 293 | 0.05M Cadmium sulfate hydrate, 0.1M HEPES, 1.0M sodium acetate trihydrate, 40% w/v 1,4-butanediol, pH 7.5, Microbatch Underoil, temperature 293K |