4O7V
SAICAR synthetase (Type-2) in complex with ADP and UDP/UMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-11-24 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 44.160, 155.120, 78.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.100 - 2.300 |
R-factor | 0.1963 |
Rwork | 0.193 |
R-free | 0.24920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u55 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.391 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.100 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.073 | 0.259 |
Number of reflections | 12230 | |
<I/σ(I)> | 20.4 | 7.1 |
Completeness [%] | 99.2 | 98.5 |
Redundancy | 6.2 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch Underoil | 4.6 | 293 | 0.2M Ammonium Sulphate, 0.1M Sodium acetate trihydrate, 30% w/v PEG monomethyl ether 2000, pH 4.6, Microbatch Underoil, temperature 293K |