4O6I

2.0A crystal structure of Lymphocytic Choriomeningitis Virus Nucleoprotein C-terminal Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-01
Detector	MARMOSAIC 300
Wavelength(s)	1.0332
Spacegroup name	P 65
Unit cell lengths	89.962, 89.962, 145.941
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.955 - 2.000
R-factor	0.2095
Rwork	0.209
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q7c
RMSD bond length	0.004
RMSD bond angle	0.639
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	38.955
High resolution limit [Å]	2.000
Number of reflections	45091
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Eppendorf Crystallization	8	277	10 mM TRIS, 300 mM NaCl, protein concentration at 1.15 mg/mL, pH 8, Eppendorf Crystallization, temperature 277K