4O56

Structure of PLK1 in complex with peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-002+
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-24
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	35.114, 69.248, 56.513
Unit cell angles	90.00, 98.63, 90.00

Refinement procedure

Resolution	24.530 - 1.800
R-factor	0.14292
Rwork	0.140
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1umw
RMSD bond length	0.020
RMSD bond angle	2.194
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.530	24.520	1.840
High resolution limit [Å]	1.800	3.870	1.800
Rmerge		0.026	0.038
Number of reflections	24743
<I/σ(I)>		36.9	4.4
Completeness [%]	99.5	99.6	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2M Ammonium sulfate, 100mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K