4O4Y

Crystal structure of the anti-hinge rabbit antibody 2095-2 in complex with IDES hinge peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-16
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	64.410, 89.370, 83.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.850
R-factor	0.1942
Rwork	0.192
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ma3
RMSD bond length	0.012
RMSD bond angle	1.368
Data reduction software	d*TREK (9.6L)
Data scaling software	d*TREK (9.6L)
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.059	0.242
Number of reflections	37290
<I/σ(I)>	17.5	6.1
Completeness [%]	89.2	51.6
Redundancy	8.9	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris, pH 8.5, 18.33% PEG3350, 0.2 M lithium sulfate, 5% isopropanol, cryoprotection: mother liquor + 20% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K