4O4X

Crystal structure of the vaccine antigen Transferrin Binding Protein B (TbpB) double mutant Tyr-167-Ala and Trp-176-Ala from Haemophilus parasuis Hp5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-07
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	239.890, 42.240, 114.020
Unit cell angles	90.00, 92.42, 90.00

Refinement procedure

Resolution	42.182 - 2.900
R-factor	0.2169
Rwork	0.216
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.840
Data scaling software	XSCALE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.182
High resolution limit [Å]	2.900
Number of reflections	26504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.08 M potassium phosphate monobasic, 18% PEG 8000, 20% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K