4O4U
Crystal structure of the vaccine antigen Transferrin Binding Protein B (TbpB) mutant Trp-176-Ala from Haemophilus parasuis Hp5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-27 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.030, 135.700, 103.960 |
| Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
| Resolution | 48.393 - 2.640 |
| R-factor | 0.2164 |
| Rwork | 0.214 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.739 |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.393 |
| High resolution limit [Å] | 2.640 |
| Number of reflections | 64684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Sodium formate, 15% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
