4O4H
Tubulin-Laulimalide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-03 |
| Detector | PSI PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.693, 156.868, 180.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.900 - 2.100 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22390 |
| Structure solution method | Difference Fourier |
| Starting model (for MR) | 4i4t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.159 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.900 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.181 | 2.890 |
| Number of reflections | 172824 | |
| <I/σ(I)> | 14.3 | 1 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 13.6 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 6% PEG, 16% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1M MES/0.1M imidazole, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
