4O45

WDR5 in complex with influenza NS1 C-terminal tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97923
Spacegroup name	P 21 21 2
Unit cell lengths	81.218, 85.830, 40.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.940 - 1.870
R-factor	0.1763
Rwork	0.174
R-free	0.21210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unpublished crystal structure of WDR5
RMSD bond length	0.013
RMSD bond angle	1.393
Data reduction software	XDS
Data scaling software	Aimless (0.2.12)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.940	37.940	1.910
High resolution limit [Å]	1.870	8.970	1.870
Rmerge	0.064	0.011	1.054
Total number of observations		1466	11120
Number of reflections	24106
<I/σ(I)>	21.7	69.8	2.3
Completeness [%]	100.0	97.9	99.9
Redundancy	7.1	5.5	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	291	25% PEG3350, 0.1 M ammonium sulfate, 0.1 M bis-tris, pH 6.5, temperature 291K