4O45
WDR5 in complex with influenza NS1 C-terminal tail
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.218, 85.830, 40.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.940 - 1.870 |
| R-factor | 0.1763 |
| Rwork | 0.174 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | unpublished crystal structure of WDR5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.393 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.12) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.940 | 37.940 | 1.910 |
| High resolution limit [Å] | 1.870 | 8.970 | 1.870 |
| Rmerge | 0.064 | 0.011 | 1.054 |
| Total number of observations | 1466 | 11120 | |
| Number of reflections | 24106 | ||
| <I/σ(I)> | 21.7 | 69.8 | 2.3 |
| Completeness [%] | 100.0 | 97.9 | 99.9 |
| Redundancy | 7.1 | 5.5 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 291 | 25% PEG3350, 0.1 M ammonium sulfate, 0.1 M bis-tris, pH 6.5, temperature 291K |
