4O3O
Crystal structure of human polymerase eta inserting datp opposite an 8-oxog containing dna template
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.07810 |
Spacegroup name | P 61 |
Unit cell lengths | 98.567, 98.567, 81.555 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.681 - 1.698 |
R-factor | 0.174 |
Rwork | 0.172 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ecq |
RMSD bond length | 0.011 |
RMSD bond angle | 1.409 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER ((MR)) |
Refinement software | PHENIX ((phenix.refine: dev_1551)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.220 | 1.730 |
High resolution limit [Å] | 1.698 | 2.140 | 1.700 |
Rmerge | 0.064 | 0.160 | 0.959 |
Number of reflections | 49366 | ||
<I/σ(I)> | 28.474 | 13.211 | 1.75 |
Completeness [%] | 99.5 | 99.4 | 99 |
Redundancy | 7.4 | 7.6 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1M MES pH 5.5, 5mM magnesium chloride, 15% PEG 2000 MME , VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |