4O3M
Ternary complex of Bloom's syndrome helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.930, 164.680, 50.980 |
| Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
| Resolution | 35.910 - 2.300 |
| R-factor | 0.1829 |
| Rwork | 0.181 |
| R-free | 0.21530 |
| Structure solution method | MAD |
| Starting model (for MR) | 2v1x |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.910 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.030 | 0.695 |
| Number of reflections | 36392 | |
| <I/σ(I)> | 18.4 | 2.1 |
| Completeness [%] | 98.8 | 99 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 6-10% Isopropanol, 50mM MES, 10mM magnesium chloride., pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
