4O2W
Crystal structure of the third RCC1-like domain of HERC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.383, 70.902, 105.549 |
| Unit cell angles | 70.93, 81.26, 90.84 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.1817 |
| Rwork | 0.181 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kci |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.292 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER (2.5.5) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.165 | 0.525 |
| Number of reflections | 97126 | |
| <I/σ(I)> | 7.1 | 1.53 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 20% PEG 1500, 0.2 M Magnesium Choride, 0.1 M Hepes pH7.5, vapor diffusion hanging drop, temperature 291K |
