4O2P
Kinase domain of cSrc in complex with a substituted pyrazolopyrimidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2011-08-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.978600 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.215, 63.248, 74.813 |
| Unit cell angles | 101.35, 90.44, 90.07 |
Refinement procedure
| Resolution | 43.340 - 2.100 |
| R-factor | 0.1974 |
| Rwork | 0.197 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oiq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.142 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 | |
| High resolution limit [Å] | 2.100 | 16.000 | 2.100 |
| Rmerge | 0.044 | 0.023 | 0.201 |
| Number of reflections | 42544 | ||
| <I/σ(I)> | 11.85 | 29.11 | 3.95 |
| Completeness [%] | 96.1 | 92.2 | 95.4 |
| Redundancy | 1.92 | 1.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% ETHYLENE GLYCOL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
