4O0F
Crystal structure of the human L-asparaginase protein T219A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 |
| Unit cell lengths | 59.471, 59.471, 299.241 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.590 - 1.920 |
| R-factor | 0.16719 |
| Rwork | 0.165 |
| R-free | 0.21481 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4GDV |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.741 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.040 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 43516 | |
| <I/σ(I)> | 11.78 | 1.86 |
| Completeness [%] | 96.3 | 87.6 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.2-2.5 M sodium Malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
