4NZ6

Steroid receptor RNA Activator (SRA) modification by the human Pseudouridine Synthase 1 (hPus1p): RNA binding, activity, and atomic model

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	273
Detector technology	PIXEL
Collection date	2013-04-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	71.420, 75.120, 110.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.997 - 2.000
R-factor	0.1856
Rwork	0.184
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.633
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.950
High resolution limit [Å]	1.900	8.500	1.900
Number of reflections	47463
Completeness [%]	99.8	98.5	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	28% PEG 3350/glycerol, 0.1M Bicine/TRIS (pH 8.5), 10% amino acids mixture, VAPOR DIFFUSION, HANGING DROP, temperature 277K