4NYT
L-Ficolin Complexed to Phosphocholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97887 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 96.130, 96.130, 139.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.950 - 2.250 |
| R-factor | 0.21439 |
| Rwork | 0.213 |
| R-free | 0.24293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.898 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
| Number of reflections | 38356 | ||
| Completeness [%] | 99.5 | 80.9 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 15%(w/v) PEG 8000, 200mM Ca acetate, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, temperature 293K |
