4NWG
Crystal structure of the tyrosine phosphatase SHP-2 with E139D mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-28 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.320, 212.420, 92.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.640 - 2.450 |
R-factor | 0.2815 |
Rwork | 0.280 |
R-free | 0.32580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.114 | 0.571 |
Number of reflections | 41625 | |
<I/σ(I)> | 9.19 | 2.31 |
Completeness [%] | 99.7 | 98.1 |
Redundancy | 6.51 | 6.28 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | 14.5% PEG4000, 0.1M Tris buffer, 0.02M DDT, pH 8.0, VAPOR DIFFUSION, temperature 293K |