4NVP
Structure of the cyclic nucleotide-binding domain of HCN4 channel complexed with 7-CH-cAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97372 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 96.010, 96.010, 50.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.470 - 2.500 |
| R-factor | 0.20117 |
| Rwork | 0.198 |
| R-free | 0.27031 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3U11_A |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.504 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.170 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.450 |
| Number of reflections | 8568 | |
| <I/σ(I)> | 16.4 | 4.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 8.1 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | HCN4 521-723 (10 mg mL-1) and 7-CH-cAMP 0.5mM were mixed in a 2:1 ratio with 100 mM TrisCl pH 8.0, 20 % PEG4000 (w/v), VAPOR DIFFUSION, SITTING DROP, temperature 277K |
