4NV1
Crystal structure of a 4-N formyltransferase from Francisella tularensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-12 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 |
Unit cell lengths | 71.942, 80.065, 109.852 |
Unit cell angles | 71.72, 88.62, 89.78 |
Refinement procedure
Resolution | 29.960 - 2.100 |
R-factor | 0.19536 |
Rwork | 0.191 |
R-free | 0.27038 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 2.293 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 | 0.231 |
Number of reflections | 121975 | |
<I/σ(I)> | 24 | 3.9 |
Completeness [%] | 90.2 | 82.9 |
Redundancy | 2.1 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 12-15% PEG8000, 100 mM HEPPS, 5 mM dTDP-Qui4NFo, 5 mM N5-THF, VAPOR DIFFUSION, HANGING DROP, temperature 293K |