4NV0
Crystal structure of cytosolic 5'-nucleotidase IIIB (cN-IIIB) bound to 7-methylguanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.670, 99.110, 74.120 |
| Unit cell angles | 90.00, 90.99, 90.00 |
Refinement procedure
| Resolution | 41.194 - 1.650 |
| R-factor | 0.1608 |
| Rwork | 0.159 |
| R-free | 0.19420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g08 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.580 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.670 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.053 | 0.547 |
| Number of reflections | 76459 | |
| <I/σ(I)> | 15.5 | 2.17 |
| Completeness [%] | 94.5 | 81.7 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 26% PEG 4000, 0.1M HEPES, 0.2M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
