4NTX
Structure of acid-sensing ion channel in complex with snake toxin and amiloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 151.560, 151.560, 123.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.000 - 2.270 |
| R-factor | 0.2072 |
| Rwork | 0.206 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1402)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.250 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.440 | |
| Number of reflections | 71494 | |
| <I/σ(I)> | 1.15 | |
| Completeness [%] | 95.3 | 62 |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 50 mM sodium acetate, 22-25% PEG 400, 10 mM magnesium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
