4NTK
QueD from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-01-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.070, 112.595, 115.094 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.340 - 1.600 |
| R-factor | 0.1866 |
| Rwork | 0.185 |
| R-free | 0.21743 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.725 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.400 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.033 | 0.546 |
| Number of reflections | 98203 | |
| <I/σ(I)> | 18.1 | 2.1 |
| Completeness [%] | 98.4 | 97 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | Crystals grown in 3% PEG4000, 0.1 M sodium acetate, pH 4.6. Crystal transfered to 35% PEG8000 and soaked for 1 hour with 7 mM sepiapterin, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
