4NRP
Crystal structure of human ALKBH5 in complex with N-oxalylglycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2013-05-09 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.555, 56.555, 143.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.19717 |
| Rwork | 0.196 |
| R-free | 0.21765 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nrm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.117 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 22530 | |
| Completeness [%] | 97.0 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 289 | 20% PEG 4K, 100mM CH3CO2NH4, 100mM Na-citrate , pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
