4NR1

Factor inhibiting HIF-1 alpha in complex with consensus ankyrin repeat domain-(d)allyl-GLY peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91730
Spacegroup name	P 41 21 2
Unit cell lengths	86.370, 86.370, 148.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.690 - 2.680
Rwork	0.164
R-free	0.20960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.690	2.750
High resolution limit [Å]	2.680	2.680
Total number of observations		12775	12453
Number of reflections	16485
<I/σ(I)>	17.3	2.9
Completeness [%]	99.9	99.9
Redundancy	10	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K