4NQT
anti-parallel Fc-hole(T366S/L368A/Y407V) homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-09-23 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.468, 44.478, 68.895 |
| Unit cell angles | 90.00, 106.86, 90.00 |
Refinement procedure
| Resolution | 37.900 - 2.100 |
| R-factor | 0.19824 |
| Rwork | 0.194 |
| R-free | 0.25284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.085 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 12855 | |
| Completeness [%] | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 25% PEG1500, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
