4NPP
The GLIC-His10 wild-type structure in equilibrium between the open and locally-closed (LC) forms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.554, 127.618, 185.807 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.160 - 3.350 |
| R-factor | 0.2284 |
| Rwork | 0.227 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hfi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.400 | 3.530 | 3.530 |
| High resolution limit [Å] | 3.350 | 3.370 | 3.350 |
| Rmerge | 0.101 | 0.983 | 1.040 |
| Number of reflections | 39475 | ||
| <I/σ(I)> | 12.9 | 2 | 1.9 |
| Completeness [%] | 99.8 | 99.9 | 99.9 |
| Redundancy | 5.2 | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 15-20% PEG2kmme, 50mM NiCl2, 0.1M Na Acetate pH4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
