4NOV

Xsa43E, a GH43 family enzyme from Butyrivibrio proteoclasticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-08-05
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	59.100, 76.520, 82.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.080 - 1.330
R-factor	0.17786
Rwork	0.177
R-free	0.18881
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.222
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.4.0077)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.200
High resolution limit [Å]	1.330
Number of reflections	82628
Completeness [%]	95.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.4	291	20% PEG8000, 0.2M NaCl, 0.1M Phosphate Citrate buffer , pH 3.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K