4NN7
Cytokine receptor complex - Crystal form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.968600 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.789, 50.105, 249.864 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.127 - 3.775 |
R-factor | 0.277 |
Rwork | 0.277 |
R-free | 0.28650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nn5 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.872 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.000 |
High resolution limit [Å] | 3.770 | 3.770 |
Rmerge | 0.267 | 0.778 |
Number of reflections | 4968 | |
<I/σ(I)> | 9.17 | 3 |
Completeness [%] | 99.5 | 97.7 |
Redundancy | 11.9 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 25% pentaerythritol ethoxylate, 0.1 M Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |