4NN5
Cytokine receptor complex - Crystal form 1A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976293 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 150.060, 79.756, 52.138 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.250 - 1.898 |
R-factor | 0.1713 |
Rwork | 0.170 |
R-free | 0.20090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3di2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.218 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.250 | 2.010 |
High resolution limit [Å] | 1.898 | 1.898 |
Rmerge | 0.086 | 0.670 |
Number of reflections | 50292 | |
<I/σ(I)> | 19.9 | 3.9 |
Completeness [%] | 99.7 | 98.4 |
Redundancy | 13.2 | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 293 | 14% PEG4000, 0.3 M sodium acetate, pH 4.5, 0.3 M calcium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |