4NN0

Crystal structure of the C1QTNF5 globular domain in space group P63

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	93
Detector technology	PIXEL
Collection date	2012-07-11
Detector	DECTRIS PILATUS
Wavelength(s)	0.9792
Spacegroup name	P 63
Unit cell lengths	50.603, 50.603, 268.531
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	134.265 - 1.420
R-factor	0.10622
Rwork	0.104
R-free	0.14168
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f3j
RMSD bond length	0.012
RMSD bond angle	1.645
Data reduction software	DPS
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	134.270	134.270	1.500
High resolution limit [Å]	1.420	4.490	1.420
Rmerge	0.041	0.036	0.122
Number of reflections	72440
<I/σ(I)>	30.3	56	8.4
Completeness [%]	99.1	99.9	93.8
Redundancy	8.2	8.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	2 M ammonium sulfate, 0.1 M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K