4NMN
Aquifex aeolicus replicative helicase (DnaB) complexed with ADP, at 3.3 resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 195.707, 195.707, 195.707 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.193 - 3.301 |
| R-factor | 0.2382 |
| Rwork | 0.236 |
| R-free | 0.27540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.163 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.200 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.116 | 0.554 |
| Number of reflections | 18616 | |
| <I/σ(I)> | 8.9 | 2.5 |
| Completeness [%] | 98.4 | 85.1 |
| Redundancy | 5.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 100mM Hepes pH 7.5, 200-300mM KSCN or NaSCN and 18-22% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
