4NM6
Crystal structure of TET2-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792, 1.2818 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.296, 88.186, 262.962 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.805 - 2.026 |
| R-factor | 0.2044 |
| Rwork | 0.202 |
| R-free | 0.24280 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.294 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.066 | 0.488 |
| Number of reflections | 34839 | |
| <I/σ(I)> | 11.86 | 3.3 |
| Completeness [%] | 93.8 | 89.49 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 277 | 23% PEG2000 MME, 0.1 M MES, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
