4NLY
Poliovirus Polymerase - C290E Loop Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-20 |
| Detector | NOIR-1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 128.802, 128.802, 111.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.810 - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ra6 |
| Data reduction software | d*TREK (9.4L) |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.810 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.074 | 0.370 |
| Number of reflections | 85946 | |
| <I/σ(I)> | 14.6 | 5 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 7.07 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 289 | Grown in sodium acetate, cacodylate, DTT. Transferred to 250 mM sodium acetate, 30% (w/v) PEG-400, 0.1 M cacodylic acid and 2 mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
