4NLT
Poliovirus Polymerase - S291P Loop Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-06 |
Detector | NOIR-1 |
Wavelength(s) | 1.000 |
Spacegroup name | P 65 |
Unit cell lengths | 129.376, 129.376, 111.931 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
Rwork | 0.222 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | d*TREK (9.4L) |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 29.940 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.180 | 0.098 | 0.358 |
Total number of observations | 28167 | 25007 | |
Number of reflections | 36788 | ||
<I/σ(I)> | 7.3 | 16.6 | 2.7 |
Completeness [%] | 99.9 | 99.6 | 100 |
Redundancy | 7.2 | 7.34 | 6.76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Hanging drop | 7 | 289 | Grown in sodium acetate, cacodylate, DTT. Transferred to 250 mM sodium acetate, 30% (w/v) PEG-400, 0.1 M cacodylic acid (pH 7.0) and 2 mM DTT, Hanging drop, temperature 289K |