4NLO
Poliovirus Polymerase - C290I Loop Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-31 |
Detector | NOIR-1 |
Wavelength(s) | 1.000 |
Spacegroup name | P 65 |
Unit cell lengths | 128.970, 128.970, 111.860 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
Rwork | 0.226 |
R-free | 0.25140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ra6 |
Data reduction software | d*TREK (9.4L) |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 29.860 | 2.280 |
High resolution limit [Å] | 2.200 | 4.730 | 2.200 |
Rmerge | 0.094 | 0.032 | 0.443 |
Total number of observations | 41474 | 36675 | |
Number of reflections | 52853 | ||
<I/σ(I)> | 13.2 | 38 | 4.4 |
Completeness [%] | 98.6 | 99.9 | 97.2 |
Redundancy | 7.36 | 7.59 | 6.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | Grown in sodium acetate, cacodylate, DTT. Transferred to 250 mM sodium acetate, 30% (w/v) PEG-400, 0.1 M cacodylic acid and 2 mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |