4NL9
Crystal structure of the human Anks3-SAM/Anks6-SAM heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 47.700, 108.520, 101.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.870 - 1.500 |
R-factor | 0.1823 |
Rwork | 0.180 |
R-free | 0.20480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nj8 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.944 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.540 |
High resolution limit [Å] | 1.500 | 6.700 | 1.500 |
Rmerge | 0.062 | 0.039 | 0.371 |
Number of reflections | 42244 | 508 | 3042 |
<I/σ(I)> | 14.83 | 30.48 | 4.16 |
Completeness [%] | 98.7 | 91.4 | 96.9 |
Redundancy | 5.32 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapor diffusion, hanging drop, streak seeding | 8 | 298 | 31.5% PEG-4000, 100mM Tris, 0.25M magnesium chloride, pH 8.0, vapor diffusion, hanging drop, streak seeding, temperature 298K |